CHEM 352—Biochemistry II—Spring 2025
PCR: The Polymerase Chain Reaction
Laboratory Document
PCR: The Polymerase Chain Reaction
Related Protocols
Densitometry
Gel Documentation
PCR Products to Gel
Related Articles
SantaLucia J. 1998. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc Natl Acad Sci USA. 95: 1460-5.
Other Documents, Links and Tools
λ Chromosome—FASTA Format
100 bp ladder
Data Folder
E-Gel Instructions
Laboratory Rotation Matrix
pBR322—FASTA Format
PCR Worksheet
Primer-BLAST
Roth lab primer design guidelines
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