The intial velocity kinetics of enzyme X were measured.
Those data are available here. Plot the data as initial velocity
vs. substrate concntration and fit the Michaelis-Menten equation to these data to extract the values of V
MAX and K
M. Compare the fitted values to the estimated values from the plot. The concentration of enzyme X in each reaction was 125 nM. Calculate k
cat and k
cat/K
M for enzyme X. Is enzyme X a "good" enzyme?
The values of VMAX is 16.6 μmoles•s-1 and the value of KM is 14.0 μM. The Michaelis–Menten plot is shown below. The value of kcat is 133 s-1 and the value of kcat/KM is 9.49 x 106 M-1•s-1. As far as enzymes go, enzyme X is rather good at catalysis under these conditions.
