Ligand Binding
[1] Relationship between Affinity and Dissociation Constant
Lehninger chapter 5, page 183, question 1
Show Answer
See answer in Lehninger on page AS–5.
[2] Reversible Ligand Binding I
Lehninger chapter 5, page 184, question 4
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See answer in Lehninger on page AS–5.
[3] Reversible Ligand Binding II
Lehninger chapter 5, page 184, question 5
Show Answer
See answer in Lehninger on page AS–5.
[4] Reversibe (but Tight) Binding to an Antibody
Lehninger chapter 5, page 184, question 11
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See answer in Lehninger on page AS–6.
[5] One Ligand Binding Site
The binding of ligand X by protein A was studied.
Data for the fraction of binding sites occupied (θ) as a function of ligand X concentration were collected. Analyze these data by nonlinear least-squares fitting to determine the value of the dissociation constant (K
d) for this interaction.
Show Answer
The value of Kd is 0.42 μM. The fitted data are shown below.
[6] Cooperative Binding
The binding of ligand Y by protein B was studied. A sigmoidal curve was observed for θ = ƒ([L]). Analyze
these data by nonlinear least-squares fitting to determine the dissociation constant (K
d) for this interaction and the number of binding sites for ligand Y on protein B.
Show Answer
The value of Kd is 0.38 μM and there are two (2) binding sites for ligand Y. The fitted data are shown below.
