CHEM 351—Biochemistry I—Fall 2026
Data Analysis with Spreadsheets
[1] Nonlinear Least-Squares Fitting
Nonlinear least-squares fitting is used to fit a nonlinear equation to a data set. Accompanying this problem is a hypothetical data set that appears to be a hyperbolic curve. Hyperbolic curves are described by equations of the form:
$$Y = {{a \times X} \over {b + X}}$$
Fit the equation above to these data calculating values for a and b as well as the correlation coefficient, r2.
Show Answer
The values of a and b are 0.260 and 1.971, respectively. The value of the correlation coefficient r2 is 0.96. A example of the spreadsheet used to calculate these values is shown below.
Screen shot of Excel with a solution to this problem
The equations for key cells are as follows:

Cell        Equation
C2        =($H$1*A2)/($H$2+A2)
D2        =(C2-B2)^2
D24        =SUM(D2:D22)
E2        =(C2-AVERAGE($B$2:$B$22))^2
F2        =(B2-AVERAGE($B$2:$B$22))^2
H4        =SUM(E2:E22)/SUM(F2:F22)
Last updated: 02 September, 2025.
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