[kevshaw@451]$ history
     1  date
     2  ls
     3  cd pcr
     4  ls
     5  less primers
     6  blastn -db human_genomic -word_size 7 -evalue 1000 -soft_masking false -query primers | less
     7  wget https://yea4.us/451/data/tgif2ly
     8  nano tgif2ly
     9  primer3 tgif2ly
    10  primer3 tgif2ly | grep 4_SEQUENCE
    11  primer3 tgif2ly | grep 4_SEQUENCE > tgif2ly_primers
    12  nano tgif2ly_primers
    13  blastn -db human_genomic -word_size 7 -evalue 1000 -soft_masking false -query tgif2ly_primers | less
    14  cd
    15  mkdir seq
    16  cd seq
    17  wget http://ftp.ensembl.org/pub/current_fasta/saccharomyces_cerevisiae/dna/Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa.gz
    18  gzip -dv Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa.gz
    19  ls
    20  ls -l
    21  less Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa
    22  grep '^>' Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa
    23  mv Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa yeast.fa
    24  ls -l
    25  samtools faidx yeast.fa
    26  ls
    27  cat yeast.fa
    28  cat yeast.fa.fai
    29  bwa index yeast.fa
    30  wget https://ftp.sra.ebi.ac.uk/vol1/fastq/SRR507/SRR507778/SRR507778_1.fastq.gz
    31  wget https://ftp.sra.ebi.ac.uk/vol1/fastq/SRR507/SRR507778/SRR507778_2.fastq.gz
    32  ls
    33  gzip -dv *.gz
    34  ls -l
    35  less SRR507778_1.fastq
    36  head -20000 SRR507778_1.fastq > 1.fastq
    37  ls -l
    38  head -20000 SRR507778_2.fastq > 2.fastq
    39  cd
[kevshaw@451]$