Thursday, 17 November, 2022
[kevshaw@451]$ history
1 ls
2 cd pcr
3 ls
4 less primer1
5 cd
6 less pcr/primer1
7 mkdir seq
8 cd seq/
9 ls
10 cd
11 mkdir dnaseq
12 cd dnaseq/
13 wget http://ftp.ensembl.org/pub/current_fasta/saccharomyces_cerevisiae/dna/Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa.gz
14 ls
15 gzip -dv Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa.gz
16 ls -l
17 mv Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa yeast.fa
18 ls -l
19 less yeast.fa
20 grep '>' yeast.fa
21 samtools
22 samtools | less
23 samtools
24 samtools faidx yeast.fa
25 ls
26 less yeast.fa
27 less yeast.fa.fai
28 cat /proc/cpuinfo
29 bwa index yeast.fa
30 wget wget http://ftp.sra.ebi.ac.uk/vol1/fastq/SRR507/SRR507778/SRR507778_1.fastq.gz
31 ftp ftp.sra.ebi.ac.uk
32 ls -l
33 gzip -dv *.gz
34 ls -l
35 less SRR507778_1.fastq
36 tail -4 SRR507778_1.fastq
37 head -20000 SRR507778_1.fastq > 1.fastq
38 ls -l
39 head -20000 SRR507778_2.fastq > 2.fastq
40 ls -l
41 bwa mem yeast.fa 1.fastq 2.fastq > yeast.sam
42 ls
43 less yeast.sam
44 samtools sort -O BAM -T . -l 0 -o yeast.bam yeast.sam
45 ls -l
46 less yeast.bam
47 samtools view yeast.bam | less
48 cd
49 .holidaze/chanukah
[kevshaw@451]$