Tuesday, 1 December, 2020
[kevshaw@451]$ history
1 ls .h*
2 .holidaze/chanukah
3 clear
4 bcftools
5 bcftools | less
6 ls
7 cd s
8 ls
9 rm 1.1*
10 rm 1.2*
11 ls
12 rm 2.1*
13 ls
14 rm 2.2*
15 ls
16 rm final*
17 ls
18 rm full.sam
19 ls
20 ls -al
21 cd
22 clear
23 reagan
24 cd s
25 las
26 ls
27 ls -l
28 ftp ftp.ncbi.nlm.nih.gov
29 ls -l
30 less snps.vcf
31 mkdir quick
32 cp 1.fastq quick/
33 cp 2.fastq quick/
34 cp yeast.fa quick/
35 cd quick
36 ls
37 bwa index yeast.fa
38 bwa mem yeast.fa 1.fastq 2.fastq > yeast.sam
39 ls -l yeast.sam
40 less yeast.sam
41 samtools sort -O BAM -T . -l 0 -o yeast.bam yeast.sam
42 ls -l
43 bcftools mpileup -Ou -f yeast.fa yeast.bam | bcftools call -vmO z -o snsp.vcf.gz
44 ls -l
45 gzip -dv snsp.vcf.gz
46 less snsp.vcf
47 history
48 nano snpcall
49 chmod x snpcall
50 ls -l
51 rm *.vcf
52 rm yeast.bam
53 rm yeast.fa.a*
54 rm yeast.fa.[a-z]*
55 ls
56 ls -al
57 rm yeast.sam
58 ls
59 ./snpcall yeast.fa 1.fastq 2.fastq
60 ls -l
61 cat snpcall
62 rm yeast.fa.[a-z]*
63 ls -l
64 cd
[kevshaw@451]$