CHEM 352—Biochemistry II—Spring 2025
Lipids
01/13
Chapter 10
Suggested Problems—lipids
Course Introduction
Fatty Acid Oxidation
01/15
Chapter 17
Suggested Problems—fatty-acid-oxidation
β–oxidation film
Fatty Acid Oxidation (continued)
01/17
Chapter 17
Diseases of Fatty Acid Metabolism
01/20
Chapter 17
Lipid Biosynthesis
01/22
Chapter 21
Suggested Problems—lipid-biosynthesis
Lipid Biosynthesis (continued)
01/24
Chapter 21
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End: 18:51
Length: 7:46
Hit-and-run epigenome editing
Biological Membranes
01/27
Chapter 11, p. 385–95
Singer SJ and Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes Science 175 720–31
Suggested Problems—biological-membranes
Membrane Dynamics
01/29
Chapter 11, p. 395–402
Membrane Slides
Membrane Transport
01/31
Chapter 11, p. 402–27
Aschroft FM (2006) From molecule to malady Nature 440 440–7
The Nucleotides
02/03
Chapter 8, p. 281–7
Suggested Problems—nucleotides
Nucleotide Biosynthesis
02/05
Chapter 22, p. 910–20
Nucleotide Degradation
  **final topic in unit I
02/07
Chapter 22, p. 920–5
DNA Structure
02/10
Chapter 8, p. 287–93
Suggested Problems—dna-structure
podcast icon What Rosalind Franklin truly contributed to the discovery of DNA’s structure
DNA Figures
DNA Structure (continued)
02/12
Chapter 8, p. 287–93
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End: 10:37
Length: 9:47
Related Article: Designer curvature
Designer curvature
RNA Structure
02/14
Chapter 8, p. 293–7
RNA Figures
Nucleic Acid Chemistry
02/17
Chapter 8, p. 297–308
SantaLucia J (1998) A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics PNAS 95 1460–5
Suggested Problems—dna-thermodynamics
Genomes
02/19
Chapter 24, p. 979–85
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Start: 0:49
End: 12:30
Length: 11:41
Waiting for the Revolution
UCSC Genome Browser
ENSEMBL Genome Browser
NCBI Genome Browser
(m) Local Alignment
02/21
Suggested Problems—local-alignment
BLAST @ NCBI
DNA Packing
02/24
Chapter 24, p. 985–1003
Suggested Problems—dna-packing
DNA Packing Figures
(m) We be GELlin'
02/26
Chapter 3, p. 92–96
Yakhnin AV, Yakhnin H and Babitzke P (2012) Gel mobility shift assays to detect protein-RNA interactions Methods Mol Biol 905201-11
Gygi SP, Corthals GL, Zhang Y, Rochon Y and Aebersold R (2000) Evaluation of two-dimensional get electrophoresis–based proteome analysis technology PNAS 97 9390-5
Leblanc, B and Moss, T (2001). DNase I footprinting. In: Moss, T (eds) DNA-Protein Interactions Methods in Molecular Biology 148 Humana Press
DNA Replication
02/28
Chapter 25, p. 1011–27
Suggested Problems—dna-replication
American Scientists postage stamps, issued 16 June, 2001
DNA Polymerase Figures
No Class—Spring Recess
03/03
 No Class—Spring Recess
03/05
No Class—Spring Recess
03/07
(m) DNA Sequencing
03/10
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Start: 16:44
End: 23:01
Length: 6:16
The future of DNA sequencing
Suggested Problems—dna-sequencing
(m) Polymerase Chain Reaction
03/12
Chapter 9, p. 327–331
(m) Polymerase Chain Reaction (continued)
  **final topic in unit II
03/14
Chapter 9, p. 327–331
Suggested Problems—polymerase-chain-reaction
Primer Blast
DNA Repair
03/17
Chapter 25, p. 1027–38
DNA Recombination
03/19
Chapter 25, p. 1038–52
DNA–Dependent RNA Synthesis
03/21
Chapter 26, p. 1057–69
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End: 10:40
Length: 9:46
Related Article: A GPS for Navigating DNA
A GPS for Navigating DNA
RNA Processing
03/24
Chapter 26, p. 1069–85
Suggested Problems—rna-processing
Protein Synthesis
03/26
Chapter 27, p. 1103–39
Suggested Problems—protein-synthesis
Protein Synthesis (continued)
03/28
Chapter 27, p. 1103–39
Protein Targeting and Degradation
03/31
Chapter 27, p. 1139–49
Suggested Problems—protein-degradation
(m) Recombinant Protein Expression
04/02
Recombinant Protein Expression Figures
(m) Absorbance Spectroscopy
04/04
Box 3, p. 80
Suggested Problems—absorbance
(m) Emission Spectroscopy
04/07
Chapter 19, p. 771–6
Box 12–3, p. 448–9
(m) Nuclear Magnetic Resonance of Proteins
04/09
Box 4–5, p. 134–6
(m) Nuclear Magnetic Resonance of Proteins (continued)
  **final topic in unit III
04/11
Box 4–5, p. 134–6
Suggested Problems—nuclear-magnetic-resonance
(m) X-ray Crystallography of Proteins
04/14
Box 4–5, p. 134–6
(m) X-ray Crystallography of Proteins (continued)
04/16
Box 4–5, p. 134–6
No Class—Easter Recess
04/18
No Class—Easter Recess
04/21
Protein Folding
04/23
Chapter 4, p. 143–151
(m) Equilibrium Studies of Protein Folding
04/25
Chapter 4, p. 143–151
Suggested Problems—protein-folding
(m) Equilibrium Studies of Protein Folding (continued)
04/28
Gassner NC, Baase WA, Mooers BHM, Busam RD, Weaver LH, Lindstrom JD, Quillin ML and Matthews BW (2003) Multiple methionine substitutions are tolerated in T4 lysozyme and have coupled effects on folding and stability Biophys Chem 100
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End: 13:14
Length: 13:14
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Diseases of Protein Folding
04/30
Reynaud E (2010) Protein misfolding and degenerative diseases Nature Ed 3 28
Final Exam—8:00
05/02
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